Acid protease Sunson® PRA100 for yeast nutrition

 PRODUCT DESCRIPTION  

Acid Protease PRA100L is produced by submerged fermentation of Aspergillus niger followed by purification and formulation. The enzyme catalyzes the hydrolysis of protein under acid condition. In alcohol fermentation industry the product will liberate more amino nitrogen and improve ethanol yield by boosting yeast’s growth, bringing economic benefits.   

MECHANISM 

The acid protease is capable of breaking down peptide bonds in proteins  in raw materials, liberating short peptides and even amino acids that are necessary for yeast’s growth. In this way ethanol yield is increased, while making the best use of raw materials and saving cost for supplementation of yeast nutrients.

 REACTION PARAMETERS 

PARAMETERS	RANGE
Activity Temperature	30℃-60℃
Optimum Temperature	40℃-50℃
Activity pH	2.5-6.0
Optimum pH	2.5-3.5

 

 

 

 PRODUCT SPECIFICATION 

Items	Description
Declared Activity*	100,000u/ml
Physical Form	Liquid
Color**	Brown
Odour	Normal microbial fermentation odour.

 *Definition of Unit: 1 unit of Acid Protease equals to the amount of enzyme, which hydrolyzes casein to get 1μg of tyrosine at 40℃ and pH3.0 in 1 min. 

** Color: Color can vary from batch to batch. Color intensity is not an indication of enzyme activity.  

PRODUCT STANDARD  

The product complies with GB1886.174. 

No.	ITEMS		INDEX
1	Bulk density/(g/ml)		1.05-1.20
2	pH/(25℃)		3.5–5.5
3	Lead/(mg/kg)		≤5.0
4	Arsenic/(mg/kg)		≤3.0
5	Total viable count/(CFU/mL)		≤50000
6	Coliform Bacteria/(CFU/mL)		≤30
7	Escherichia coli	(CFU/mL)	≤10
		(MPN/mL)	≤3.0
8	Salmonella/(25mL)		Not Detected

  APPLICATION RECOMMENDATION 

The recommended dosage is 0.05-3kg of the enzyme preparation per ton of total raw materials. The dosage has to be optimized based on each application, the raw material specifications, product expectation and processing parameters. It is better to begin the test with the convenient volume.  

SAFE HANDLING PRECAUTIONS 

Enzyme preparations are proteins that may induce sensitization and cause allergic type of symptoms in susceptible individuals. Prolonged contact may cause minor irritation for skin, eyes or nasal mucosa. Any direct contact with human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor.  

WARNINGS 

Keep sealed after use every time to avoid microbial infections and inactivation of enzymes until its finish.  

PACKAGE AND STORAGE 

Ø  Package： 25kgs/drum; 1,125kgs/drum. 

Ø  Storage： Keep sealed in a dry and cool place and avoid direct sunlight. Slight sedimentation is acceptable since it will not impact performance of the product. 

 

Ø  Shelf life:  12 months in a dry and cool place.